Document Details
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Document Type |
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Thesis |
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Document Title |
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Diversity of Swine Flu A (H1N1) Isolated from Human in Makkah Region الاختلافات الجينية لفيروسات انفلونزا الخنازير (H1N1) المعزولة من المرضى المصابين في منطقة مكة المكرمة |
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Subject |
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Flu |
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Document Language |
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English |
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Abstract |
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The H1N1 pandemic virus is a newly emergent human influenza A virus that is closely related to a number of currently circulating pig viruses in the ‘classic North American’ and ‘Eurasian’ swine influenza virus lineages. Since the first reports of the virus in humans in April 2009, H1N1 virus has spread to 168 countries and overseas territories. Saudi Arabia also witnessed severe H1N1 pandemic virus epidemic with considerable morbidity and mortality in different parts starting from June 2009. The objective of this study is molecularly diagnosis and detect the diversity of human swine influenza virus in different isolates. Our sample collected from Makkah region during July 2009 to November 2010 was confirmed through real time RT-PCR after isolation. Positive samples were further processed for RT-PCR and sequencing. The retrieved sequence aligned with the GeneBank® data to detect the variation in the sequence of swine flu different isolates. As a result, full HA and NA segments sequencing of our H1N1 isolates revealed highest homology (99%) and a good homology with other circulating novel swine flu viruses. The HA segment sequence phylogeny revealed the circulation belonging to New York, Jakarta and Scotland. The NA segment was homologous to Monagua, Chile and Helsinki. The reasons behind this homology may be cause of mixed genogroups of S-OIV in Saudi Arabia during Hajj and Umrah seasons and the presence of unidentified carriers from different countries |
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Supervisor |
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Ibrahim Hassan Kamal |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1434 AH
2013 AD |
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Number Of Pages |
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60 |
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Co-Supervisor |
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Esam Ibraheem Azhar |
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Added Date |
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Wednesday, September 11, 2013 |
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Researchers
| نورة عبدالحميد عثمان | Uthman, Norah Abdulhamed | Investigator | Master | |
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